jetprime reagent  (Promega)

Journal: Experimental and Therapeutic Medicine

Article Title: Effect of overexpression of GRN on the proliferation and osteogenic capacity of human periodontal cells

doi: 10.3892/etm.2024.12783

Figure Lengend Snippet: pLV-GRN transfection of hPDLCs. (A) Lentiviral infection screening results (magnification, x40). (B) RT-qPCR results of the relative expression of GRN in hPDLCs after transfection ( *** P<0.01). (C) Western blotting results of the relative expression of GRN in hPDLCs following transfection ( ** P<0.05). pLV, lentivirus recombinant plasmid; GRN, granulin precursor gene; hPDLCs, human periodontal ligament cells; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Upon reaching 80% confluence, cell transfection was performed using the jetPRIME Transfection Reagent Kit (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's instructions.

Techniques: Transfection, Infection, Quantitative RT-PCR, Expressing, Western Blot, Recombinant, Plasmid Preparation, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Scientific Reports

Article Title: Nrf2 depletion enhanced curcumin therapy effect in gastric cancer by inducing the excessive accumulation of ROS

doi: 10.1038/s41598-024-81375-1

Figure Lengend Snippet: Cur combined with Nrf2 knockdown effectively inhibited the proliferation, migration and invasion of GC cells. ( A , B ) rt-qPCR validated the efficiency of three siRNA sequences to knock down Nrf2 in AGS and HGC27 cells. ( C , D ) After GC cells were transfected with siNC and siNrf2 for 24 h, they were treated with Cur (0 and 20 µM) for 24 h. Cell viability was detected by the CCK8 assay. ( E – H ) The migratory capacity of GC cells was compared using a wound healing assay and the wound healing area was quantified. ( I – L ) GC cells were transfected with siNC and siNrf2 or treated with Cur (0 and 20 µM) for 24 h. Transwell assay was used to compare the migratory and invasive capacities of GC cells. Scale: 500 μm. Data are presented as the mean ± SD. n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: After incubating cells (2 × 10 5 /well) in 6-well plates for 24 h, jetPRIME in vitro siRNA transfection reagent (Yeasen, Shanghai, China) and complete media were added, along with small interfering RNAs (siRNAs) targeting Nrf2 (siNrf2) or negative control (NC) (50 nmol/l).

Techniques: Knockdown, Migration, Quantitative RT-PCR, Transfection, CCK-8 Assay, Wound Healing Assay, Transwell Assay